Characterizing spatial information of chromatin packing using the finite difference time domain (FDTD) method

In recent years, cell fate and phenotype can be affected without altering the underlying genetic sequence by the control of three-dimensional structure of chromatin packing domains. Many imaging methods have been developed to try to resolve the 3D chromatin organization. Here, we propose using the Finite Difference Time Domain (FDTD) method to compare and bridge the knowledge of spatial information regarding chromatin packing domains from different imaging modalities. We validated the ability of PWS, a label free high throughput live cell imaging method, to characterize spatial information of chromatin packing, making way for high spatial and temporal resolution chromatin imaging.