Secretory inhibitor of lysozyme and biofilm formation of intestinal strains Candida in children with reactive arthritis

This short communications compares the phenotypic properties of Candida species isolated from gut of children with reactive arthritis, such as secretory inhibitor of lysozyme and biofilm formation. A total of 65 clinical strains of Candida species isolated from the feces of healthy children and children with ReA were tested. SIL production of Candida by inhibiting lysozyme activity against Micrococcus luteus ATCC 15307 were studied (mg/mL*OD). Quantitative assessment of biofilm formation was performed using crystal violet binding assay method (CU). A significantly higher proportion of ReA strains were SIL-positive (77.8% vs. 40.0%) and BF-positive (64.4% vs. 30.0%) compared with non-ReA strains (P < 0.01). These results suggest that the functional significance of SIL production and biofilm formation must be estimated, of investigating the roles of intestinal of Candida in the pathogenesis of spondylarthritis, including reactive arthritis. It is imperative to delineate microbial factors that contribute to ReA development.