FIGURE 1 from Identification and Characterization of a Small Molecule Bcl-2 Functional Converter

Bcl-2–dependent effects of BFC1108. A, Structure of BFC1108 (5-chloro-N-(2-ethoxyphenyl)-2-[(4-methoxybenzyol)amino]benzamide). B, Left: Bcl-2 expression in MDA-MB-231 cells transfected with pcDNA control vector (MDA-MB-231/Vector) or Bcl-2 expression vector (MDA-MB-231/Bcl-2) was determined by immunoblotting. Right: Cells were exposed to BFC1108 in a medium containing 10% FBS for 48 hours and viability was determined. ***, P < 0.001. C, Left: Bcl-2 expression in MCF-7 cells transfected with pcDNA control vector (MCF-7/Vector) or Bcl-2 expression vector (MCF-7/Bcl-2) was determined by immunoblotting. Right: Cells were exposed to BFC1108 in a medium containing 10% FBS for 48 hours and viability was determined. **, P < 0.01. D, Left: Bcl-2 expression in Jurkat cells transfected with control vector (Jurkat/Vector) or Bcl-2 expression vector (Jurkat/Bcl-2) was determined by immunoblotting. Right: Cells were exposed to BFC1108 in a medium containing 10% FBS for 48 hours and viability was determined. ***, P < 0.001. E, Left: Knockdown of Bcl-2 in MDA-MB-468 cells was determined by immunoblotting. Right: Bcl-2 siRNA and control siLuc siRNA transfected MDA-MB-468 cells were treated with BFC1108 in a medium containing 10% FBS for 48 hours. *, P < 0.05.