Abstract 2348 Deployment of Quantitative Proteomics Approaches in Defining Dynamic Changes the Diel Plant Proteome

Plants carefully regulate their daily cellular and physiological processes through a combination of circadian (anticipatory) and light-responsive (reactive) mechanisms in order to adapt to their changing daily environment and to maximize growth. To date, our understanding of diel plant cell regulation has largely been driven by a combination of genetic and transcriptomic approaches, where substantial portions of protein encoding genes have been found to possess diel fluctuations in abundance, including 100's of nuclear encoded, chloroplast-targeted proteins involved in diverse biological processes. The advancements made in quantitative proteomic technologies and workflows over the past 10 years has created an exciting new frontier of discovery that now allows us to define the diel plant proteome directly. However, substantial challenges remain when considering the assessment of dynamic temporal (e.g. daily) and/or spatial (e.g. organelle) changes. Here, we discuss our efforts to overcome the challenges that exist when endeavoring to assess dynamic changes in the chloroplast proteome, and further explore quantitative proteomic approaches as a means to help us better understand how the circadian clock intersects with chloroplast cell signaling and metabolic processes.