Extended blood stage sensitivity profiles ofPlasmodium vivaxto doxycycline and tafenoquine usingPlasmodium cynomolgias a model

Testing Plasmodium vivax antimicrobial sensitivity is limited to ex vivo schizont maturation assays, which precludes determining the IC 50s of delayed action antimalarials such as doxycycline. Using Plasmodium cynomolgi as a model for P. vivax , we determined the physiologically significant delayed death effect induced by doxycycline (IC 50(96h) , 1401 ± 607 nM). As expected, IC 50(96 h) to chloroquine (20.4 nM), piperaquine (12.6 µM) and tafenoquine (1424 nM) were not affected by extended exposure.