FIGURE 5 from Cancer-associated Fibroblast–specific Expression of the Matricellular Protein CCN1 Coordinates Neovascularization and Stroma Deposition in Melanoma Metastasis

Loss of CCN1 expression from CAFs does not affect cell proliferation or apoptosis in a syngeneic model of melanoma metastasis. A, PCNA assay in (top) tumor and (bottom) stroma. Cell proliferation was detected using an anti-PCNA antibody. Representative images shown; graphs show the mean percentage of PCNA positive cells in CAFs ± SEM; CCN1f/fn = 7 and CCN1−/−n = 4, Student t test. B, TUNEL assay to detect apoptosis in stroma. Representative images shown; graphs show the mean percentage of TUNEL positive cells in stroma ± SEM, CCN1f/fn = 7 and CCN1−/−n = 4, Student t test.