FIGURE 2 from Estrogen Receptor Mutations as Novel Targets for Immunotherapy in Metastatic Estrogen Receptor–positive Breast Cancer

Kinetics of peptide: HLA-A*0201 dissociation. T2 cells were pulsed with indicated peptides for 8–12 hours, washed, and then stained for surface HLA-A*0201 expression at indicated timepoints for wildtype-1 and E380Q (A), wildtype-2 and Y537S (B), wildtype-2 and D538G (C). MFI for each timepoint was normalized to initial staining (time = 0). Linear regression analysis was used to plot lines of best fit for each individual peptide, and half-life for each peptide is shown in Table 2. Data are pooled from three independent experiments performed in triplicate.