The Possibility of the Use of Leukocytes in Weak Blood Group Antigens Detection

Introduction: Mixed agglutination reaction is used for revealing A,B and H blood group antigens on the cells. The aim of the study was to visualize the antibody binding on weak blood group antigens of leukocytes in order to improve the quality of blood typing. Material and methods: The antibodies were contacted with group A and B leukocytes at 4 C. Mixed agglutination reaction was used to reveal group antigens on leukocytes using test erythrocytes. Results: The heated anti-A,B serum after adsorption on group A leukocytes agglutinated group A erythrocytes. Anti-A,B heated plasma after the contact with group B leukocytes agglutinated group B erythrocytes. Anti-A,B serum after adsorption on group B(A+) leukocytes agglutinated group B (A+) erythrocytes. Anti-A,B serum after adsorption on group A(B+) leukocytes agglutinated group B erythrocytes. Conclusions: MAR allowed to reveal both agglutinogenic and weak adsorbing blood group antigens, that helps to perform accurate blood group typing, especially for transplantation goals. The study revealed, the detection of adsorbing antigens of leukocytes should be performed at 4 C.