The optimization of gold nanoparticles–horseradish peroxidase as peroxidase mimic using central composite design for the detection of hydrogen peroxide
Experimental analysis demonstrates sensitive hydrogen peroxide detection in beverages using gold nanoparticle conjugates, highlighting potential for portable food quality monitoring.
Key Points
Absorbance correlates strongly with hydrogen peroxide concentration with an R2 of 0.9956, yielding beverage sample recoveries between 95.51% and 118.85% on a portable platform.
Optimization using central composite design established 25 °C and pH 7 as optimal conditions, maximizing tetramethylbenzidine oxidation by gold nanoparticles and horseradish peroxidase.
Supports rapid detection of reactive oxygen species in commercial beverages, providing an accessible gold nanotechnology platform to assess oxidative stress-inducing contaminants.