The Application of DNA Origami in Biosensing

Biosensing plays a vital role in medical diagnostics, environmental monitoring, and food safety, enabling highly sensitive and specific detection of diverse biological and chemical targets. However, conventional biosensing platforms still suffer from limited sensitivity, poor nanoscale resolution, and restricted multiplexed or dynamic detection capabilities. DNA origami, as an emerging bottom-up nanofabrication strategy, enables the construction of programmable nanostructures with high spatial precision. This capability allows the rational arrangement of functional molecules at the nanoscale, thereby offering significant advantages for biosensing applications. Specifically, DNA origami can enhance signal amplification, improve spatial resolution, and enable multiplexed detection under complex conditions. In this review, we provide a systematic overview of recent advances in the application of DNA origami across various classes of biosensors, including microscopy-based biosensors, nanopore biosensors, electrochemical biosensors, fluorescent biosensors, SERS biosensors, and other related biosensors. We aim for this review to advance the development of DNA origami-based biosensing and to provide new insights for researchers working in related fields.