Single-molecule FISH uncovers mRNA expression and post-transcriptional regulation in T cells, suggesting distinct cytokine pathways.
Key Points
T-cell smFISH revealed high levels of cytokine mRNA expression, indicating a diverse transcription response among T cells.
Nuclear accumulation of cytokine-specific mRNAs was observed during activation, while cytoplasmic mRNAs underwent decay through translation-dependent mechanisms.
The study indicates that HuR selectively regulates cytokine mRNA, highlighting the complexity of post-transcriptional processes.
Using single-cell resolution, this technique offers novel insights into the dynamic regulation of mRNA in human T cells.