Background: Adipose tissue androgen turnover, dictated at least in part by the enzymes AKR1C2 and AKR1C3, has been linked to abdominal obesity. Recently, we investigated a single-nucleotide polymorphism (SNP) named rs28571858, that might increase AKR1C2 and AKR1C3 expression in human adipose tissue. Here, we studied the impact of rs28571848 on adipose tissue function and cardiometabolic health in bariatric surgery candidates. Methods: We genotyped a sample of 2776 bariatric surgery candidates and retrospectively obtained anthropometry, blood lipid and glucose profiles, menopausal status and medication use. In a subsample of 135 individuals (62% women, age 42 years, BMI of 51 kg/m2), we additionally assessed AKR1C2 and AKR1C3 expression in whole tissue by RT-qPCR. Features of adipose tissue dysfunction, such as mean adipocyte diameter and pericellular fibrosis were assessed by histological staining and semi-automated image analysis. Finally, adipose tissue AKR1C family enzyme activity was measured by fluorimetry. Results: The rs28571848 SNP affected AKR1C3 expression in both subcutaneous (SAT) and visceral adipose tissue (VAT) in women only, while not altering AKR1C2 expression in either men or women. Individuals carrying the minor allele exhibited increased VAT AKR1C activity compared to those with the wildtype genotype. Analysis of blood lipid profile in the whole cohort revealed that TT carriers had elevated total cholesterol, LDL-cholesterol, and indices of insulin resistance. Conclusion: The rs28571858 SNP increased adipose tissue AKR1C3 expression and activity in women. Increased AKR1C3 may contribute to an adipose tissue milieu that prompts lipogenesis, adversely affecting cardiometabolic health by disrupting lipid homeostasis and insulin sensitivity.
Ostinelli et al. (Tue,) studied this question.