Experimental identification of preQ₁-binding RNAs in the pathogenic bacterium Listeria monocytogenes

Q: What type of study is this?

This is a Experimental Study study.

Malou Hanisch; Laurin Flemmich; Christoph Mitteregger; Ingo Bauer; Cristian A. Velandia-Huerto; Ivo L. Hofacker; Ronald Micura; Alexandra Lusser

doi:10.1039/d5cb00102a

January 1, 2025RSC Chemical BiologyOpen Access

Experimental identification of preQ₁-binding RNAs in the pathogenic bacterium Listeria monocytogenes

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Key Points

Discovery of an mRNA regulating upstream open reading frame usage, highlighting the role of preQ1 in gene expression.
Validation of a predicted riboswitch that binds two preQ1 molecules, essential for microbial function.
In-depth pull-down and chemical analysis methods used to identify preQ1-binding RNAs within Listeria monocytogenes.
Implications suggest further exploration of regulatory mechanisms impacted by preQ1 could open new avenues in microbiology.

Implication

Experimental analysis found preQ1 riboswitch binding two molecules in Listeria monocytogenes, revealing regulatory elements.

Abstract

Pull-down, chemical, and functional analysis of preQ 1 -binding RNAs: Validation of a predicted preQ 1 riboswitch binding two preQ 1 molecules and discovery of an mRNA element regulating upstream open reading frame usage in a preQ 1 -dependent manner.

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Cite This Study

Hanisch et al. (2025) studied this question.

synapsesocial.com/papers/68f04927e559138a1a06dad9 https://doi.org/10.1039/d5cb00102a

View Full Paper

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Experimental identification of preQ₁-binding RNAs in the pathogenic bacterium Listeria monocytogenes

Key Points

Implication

Abstract

Discussion

Cite This Study

Experimental identification of preQ₁-binding RNAs in the pathogenic bacterium Listeria monocytogenes

Key Points

Implication

Abstract

Discussion

Cite This Study

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