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October 16, 2025Materials3 citationsOpen Access

Composting Poultry Feathers with Keratinolytic Bacillus subtilis: Effects on Degradation Efficiency and Compost Maturity

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JSJustyna Sobolczyk-BednarekACAnna Choińska-PulitWŁWojciech Łaba

Key Points

  • The inoculation with Bacillus subtilis accelerated compost maturation, improving nutrient availability and quality.
  • A total of 78% of feather material was solubilized within 7 days, demonstrating the efficacy of the keratinolytic strain.
  • Laboratory solid-state cultures showed double the dry mass loss in inoculated samples compared to controls, enhancing compost efficiency.
  • Inoculation specifically enhanced mineralization ratios, suggesting better compost utilization as an organic soil amendment.

Abstract

The continuous advancement of the food industry is accompanied by increased generation of animal waste, including poultry feathers. Composting presents a sustainable alternative to disposal methods such as incineration by converting waste into valuable fertilizer products. This study aimed to evaluate the impact of inoculation with the keratinolytic strain Bacillus subtilis P22 on the quality and maturity of compost produced from feathers combined with organic additives (wood shavings and lignite). The experiment involved evaluation of the keratinolytic potential of the tested strain, and characterization of its proteolytic enzymes, solid-state cultures and composting conducted at semi-technical scale. The B. subtilis P22 strain demonstrated the ability to solubilize 78% of feather material within 7 days of cultivation. The keratinolytic enzyme complex was likely dominated by polycatalytic alkaline serine proteases, i.e., subtilisins. The effectiveness of the inoculum was confirmed in laboratory solid-state cultures, where the dry mass loss in inoculated samples was twice that of the control containing only endogenous microflora. At the semi-technical scale, inoculation with B. subtilis P22 significantly accelerated compost maturation and mineralization (C/N = 10.2; N-NH4+/N-NO3− = 0.4; Cw/Corg = 0.9) compared to the control. The final compost’s mineral composition indicates its potential for use as an organic soil amendment.

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Cite This Study

Sobolczyk-Bednarek et al. (2025) studied this question.

synapsesocial.com/papers/68f0f51d8dd8ea469b1d6e83https://doi.org/10.3390/ma18204667
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