Human coronary nuclear isolation protocol v1

Q: What type of study is this?

This is a Experimental Study study.

D. Li; João Monteiro

doi:10.17504/protocols.io.yxmvmbqd5g3p/v1

October 14, 2025Open Access

Human coronary nuclear isolation protocol v1

View Full Paper

Ask AI

Bookmark

Share

Key Points

Nuclear isolation yields clean nuclei suitable for downstream applications.
The protocol emphasizes efficient lysis and debris removal for high-quality outcomes.
High-quality nuclei are preserved to maintain integrity for single-nucleus applications.
The method facilitates capture techniques such as FACS and 10x Genomics.

Implication

This protocol demonstrates nuclear isolation from human tissue, ensuring optimal quality for FACS and 10x Genomics applications.

Abstract

This protocol outlines a streamlined method for isolating high-quality nuclei from human coronary tissue for downstream single-nucleus applications. The approach ensures efficient lysis, removal of debris, and preservation of nuclear integrity, yielding clean nuclei suitable for FACS and 10x Genomics capture.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Li et al. (2025) studied this question.

synapsesocial.com/papers/68f408995de60f8893c6fd1b https://doi.org/10.17504/protocols.io.yxmvmbqd5g3p/v1

View Full Paper

Ask AI

Bookmark

Share

Human coronary nuclear isolation protocol v1

Key Points

Implication

Abstract

Discussion

Cite This Study

Human coronary nuclear isolation protocol v1

Key Points

Implication

Abstract

Discussion

Cite This Study

Keep reading

Keep reading