Application of the Kubelka-Munk model for fast intraoperative analysis of intestinal optical properties using a fiber optic spectrometer

Intraoperative determination of optical properties of biological tissues is an important task of medical physics, in particular, for photodynamic therapy, because it allows personalizing photodynamic treatment by accurately calculating the required light dose. In this work, we propose a new approach to simultaneous measurement of diffuse reflectance and transmission spectra of the colon wall tissue during intestinal anastomosis, based on the use of two fiber-optic devices to deliver broadband radiation both from the side of the intestinal lumen and from the outer side of the intestinal wall, from which diffuse scattered light is also recorded. To restore the optical properties of these tissues it was proposed to use the Kubelka-Munk model with transformation of the optical parameters of the model into the optical parameters of the diffusion approximation theory with custom equations based on the results of numerical modeling. The proposed approach have been tested ex vivo on the biological samples of GIT tissues, allowing us to conclude on its applicability in clinical conditions.