Cell Cycle Arrest by a Natural Product via G2/M Checkpoint

AAb bs st tr ra ac ct t CKBM is a natural product that exhibits a novel anti-tumor activity through the induction of cell cycle arrest and apoptosis.We have investigated its effects on cell cycle regulation using a gastric cancer cell line, AGS.The effects of CKBM on cell proliferation, cell cycle regulation and apoptosis were analyzed using BrdU (5-bromo-2'-deoxyuridine) cell proliferation assay and flow cytometric analysis, respectively.Specific cellular protein expressions were measured using Western blot analysis.Flow cytometric analysis indicated that CKBM induced G2/M cell cycle arrest and apoptosis, whereas differential protein expressions of p21, p53 and 14-3-3σ (stratifin) using Western blot analysis were enhanced.The differential expressions of p21, p53 and 14-3-3σ in AGS cancer cells after CKBM treatment may play critical roles in the G2/M cell cycle arrest that blocks cell proliferation and induces apoptosis. KKe ey y w wo or rd ds s 14-3-3σ (stratifin), G2/M arrest, cell proliferation, checkpoint protein