Kernel Dehydration Characteristics Is Related to Kernel Microstructure and Starch Granule Size Distribution in Different Maize Varieties

In the Huang-Huai-Hai region, a high kernel moisture content remains a primary constraint for the mechanical harvesting of maize kernels. Recent studies have largely focused on the relationships among ear traits, meteorological factors, and kernel dehydration. However, the regulatory mechanisms underlying the influence of kernel microstructure and starch granule size distribution on dehydration characteristics remain unclear. In this study, the fast-dehydrating variety Jingnongke 728 (JNK728) and the slow-dehydrating variety Zhengdan 958 (ZD958) were selected as experimental materials to compare the varietal differences in kernel microstructure and starch granule size distribution, and to investigate their roles in regulating kernel dehydration characteristics. The results showed that JNK728 had a significantly higher kernel dehydration rate (KDR). Compared with ZD958, JNK728 exhibited average increases of 15.22% in the pre-physiological maturity dehydration rate (pre-KDR) and 97.72% in the post-physiological maturity dehydration rate (post-KDR). The higher accumulations of kernel total starch content and amylopectin content were also observed in JNK728. Kernels of JNK728 were characterized by thinner pericarp at 35 days after pollination (DAP), lower vitreousness and a higher proportion of floury endosperm. Additionally, JNK728 displayed more uniformly sized starch granules with smooth surfaces, wider intergranular spaces, and looser starch packing. Moreover, the volume, number, and surface area of large starch granules (≥10 μm) in JNK728, increased by 2.91%, 10.94%, and 4.95%, respectively. These findings enhance the understanding of the regulatory role of kernel microstructure and starch granule size distribution in dehydration characteristics, offering theoretical guidance for the development of mechanical maize kernel harvesting technologies in the Huang-Huai-Hai region.