Synapse
⌘+K
Synapse
PulseExploreJournal ClubResearchersJournals
Instagram
HomeJournal ClubExplore
November 21, 2025npj Science of FoodOpen Access

Enhanced heme production in industrial Saccharomyces cerevisiae through metabolic engineering

View Full Paper
Ask AI
Bookmark
Share

Authors

HLHyun Jae LeeTLTAE-GI LEECCChang-Hee Cho

Discussion

Loading...

Member takes

Overview

Metabolic engineering improves heme production in Saccharomyces cerevisiae, highlighting applications in pharmaceuticals and bioenergy.

Key Points

  • To develop an efficient Saccharomyces cerevisiae-based cell factory for heme production.
  • Optimized medium composition for S. cerevisiae KCCM 12638 strain.
  • Utilized CRISPR/Cas9 for genome editing to overexpress HEM2, HEM3, HEM12, and HEM13 genes.
  • Inactivated HMX1 gene to prevent heme degradation.
  • Conducted batch and glucose-limited fed-batch fermentation.
  • Achieved a heme titer of 9 mg/L in batch fermentation, a 1.7-fold improvement over wild-type.
  • Produced 67 mg/L heme in glucose-limited fed-batch fermentation.

Cite This Study

Lee et al. (2025) studied this question.

synapsesocial.com/papers/6924e3ffc0ce034ddc34f53ahttps://doi.org/10.1038/s41538-025-00618-1
View Full Paper
Ask AI
Bookmark
Share