Experiments reveal mutations in conserved regions of SlBRC1B promoter alter bud dormancy in tomato, suggesting transcription factors are key regulators.
Key Points
To investigate how conserved regions in the SlBRC1B promoter regulate bud dormancy in tomato.
Identified conserved regions upstream of SlBRC1B using phylogenetic footprinting.
Created promoter mutants using CRISPR/Cas9 targeting each conserved region.
Conducted detailed characterization of bud outgrowth in mutants.
Screened CR4 with an axillary bud-specific cDNA library using a yeast one-hybrid assay.
Most CR mutants showed decreased bud outgrowth, indicating strong regulation of SlBRC1B.
Identification of various transcription factors including MYB, GRF, NAC, and MADS in the screening.