Smart Integration of Lyophilized B and T cell Panels in Multiomic Analysis: Enhanced Resolution and Cost Efficiency in Immune Profiling Using Microwell-based CITEseq assay 3267

Description High-throughput single-cell RNA sequencing (scRNA-seq) is essential for profiling complex cell populations. While traditional scRNA-seq captures RNA data only, advances with DNA-barcoded antibodies now enable simultaneous mRNA and protein analysis, providing a richer multiomic view. This often requires high sequencing depth, especially for large panels with redundant markers or high-density antigens monopolizing reads. Ambient unbound antibody noise during cell capture can also reduce data precision. To address these challenges, we developed 30-plex lyophilized T and B cell panels for targeted immune response profiling. These panels support marker “drop-ins” as needed, with minimal performance impact, and can be used independently or combined. Integrating essential lineage markers, the panels utilize selective signal attenuation with unconjugated antibodies to reduce sequencing demands for high-density antigens, enabling detection of low-abundance proteins and lowering sequencing costs. A microwell-based single-cell capture method, combined with post-capture buffer washes, minimizes background noise and enhances protein resolution, a clear advantage over droplet-based approaches. This modular, adaptable design provides cost-effective, high-resolution multiomic profiling, allow researchers to customize immune profiling to their needs.BD and the BD Logo are trademarks of Becton, Dickinson and Company.