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November 14, 2025International Journal of SurgeryOpen Access

Strategic engineering of DNA aptamers as precision modulators in CRISPR-driven oncogene control

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Authors

ALAolin LiCYChunyan YangZZZi-Jian Zhao

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Overview

This platform shows enhanced gene regulation and therapeutic efficacy in controlling oncogenes during cancer progression.

Key Points

  • The aim is to develop DNA aptamers that enhance CRISPR gene regulation for cancer therapy.
  • Identified five DNA aptamers specific to SaCas9 protein.
  • Conducted functional assays to assess transcriptional regulatory activity.
  • Engineered aptamers for promoter element association.
  • Delivered the system in vivo using In vivo-jetPEI® reagents.
  • Monitored tumor growth and metastasis.
  • Aptamers showed varying impacts on SaCas9 activity and gene expression control.
  • System significantly suppressed tumor growth and metastasis in vivo.
  • Aptamer-CRISPR complexes enabled logic-gated responses for gene regulation.

Cite This Study

Li et al. (2025) studied this question.

synapsesocial.com/papers/692519b4c0ce034ddc35458fhttps://doi.org/10.1097/js9.0000000000003837
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