A novel spectrophotometric assay quantifies polysaccharides in aqueous solutions, revealing extracellular polymeric substances in soil.
We present an inexpensive and practical microscale spectrophotometric acid-phenol assay to quantify polysaccharides in aqueous solution. The instant reaction within disposable materials is designed and benchmarked for use in quantifying extracellular polymeric substances (EPS) in soils. Precipitation recovery was high across expected ranges of soil EPS content.
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G et al. (2025) studied this question.
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