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This research aims to reduce sinapine content in Camelina sativa seeds using genome editing.

November 10, 2025Plant Biotechnology JournalOpen Access

Reducing the Sinapine Levels of Camelina sativa Seeds Through Targeted Genome Editing of REF1

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Genome editing lowers sinapine levels in Camelina sativa, indicating improved feed quality without sacrificing agronomic traits.

This research aims to reduce sinapine content in Camelina sativa seeds using genome editing.
Utilized CRISPR/Cas9 for targeted genome editing of REF1 in Camelina sativa.
Analyzed the effects of REF1 editing on sinapine levels and agronomic traits.
Employed ultra-performance liquid chromatography and electrospray ionisation for metabolite profiling.
Sinapine levels decreased by 56% in edited Camelina seeds.
Seed traits such as lipid and protein content remained unaffected post-editing.
Contrasting metabolite profiles were observed in seeds and leaves of ref1 mutant lines.

Kelly et al. (2025) studied this question.

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