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THRAISE provides RNA-seq analysis with quality control and differential expression in real-time, highlighting its user-friendly interface.
THRAISE (Transcriptome-wide High-throughput RNA-seq Analysis Integrated System) is a highly streamlined web platform for RNA-seq analysis that requires no programming expertise. Starting from raw NCBI SRA data, it performs the entire analysis pipeline, which includes quality control, alignment, quantification, differential expression, and functional enrichment using GO, KEGG, Reactome, and GSEA. For datasets with limited replicate (n = 1), an alternative workflow is also provided, based on transcripts per million (TPM). With real-time monitoring, interactive visualization, and a user-friendly interface, THRAISE enables accessible and reproducible RNA-seq analysis for all researchers.
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Heo et al. (2025) studied this question.
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