Single-cell analysis shows anti-CD3 treatment alters CD8+ T cells and beta cell states in type 1 diabetes, suggesting mechanisms of long-term tolerance.
Key Points
This research aims to characterize the impact of anti-CD3 mAb treatment on CD8+ T cells and beta cells in autoimmune diabetes.
Characterized single-cell transcriptome of islets from remitter NOD mice post anti-CD3 treatment.
Compared CD8+ T cells from remitted NOD mice to prediabetic controls.
Analyzed expression of specific genes in autoreactive CD8+ T cells and beta cells.
CD8+ T cells in remitted NOD mice exhibit a more diverse repertoire.
CD8+ T cells showed a naïve-like differentiation trajectory relative to controls.
Islet beta cells underwent remodeling towards a more differentiated state.