Identification of Bos taurus and Bos grunniens based on SNP

The article examined samples of domestic yak and three breeds of cattle to assess the differentiating potential of the polymorphic variants Chr4:68609356G>T (JAZF1 gene), Chr14:35695388G>T (SLCO5A1 gene) and Chr19:63181970C>G (CEP112 gene). The high accuracy (99.67%), specificity (100%) and sensitivity (100%) of the proposed test model consisting of these three polymorphisms for the identification of domestic yaks and cattle were confirmed. A fast and simple identification method has been developed based on this model using competitive allele-specific PCR (KASP) technology, which can significantly reduce the time and financial costs of molecular genetic analysis, as well as reduce the risk of cross-contamination of samples.