Optimization of the genetic code expansion technology for intracellular labelling and single-molecule tracking of proteins in genomically re-coded E. coli

Filip Ilievski; Linnea Wikström; Anneli Borg; Ivan L. Volkov; Gerrit Brandis; Magnus Johansson

doi:10.1039/d5cb00221d

January 1, 2025RSC Chemical BiologyOpen Access

Optimization of the genetic code expansion technology for intracellular labelling and single-molecule tracking of proteins in genomically re-coded E. coli

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Key Points

Single-molecule tracking enables real-time analysis of proteins in living cells, enhancing understanding of cellular interactions.
Research highlights the role of advanced labelling techniques like dye-labelled SNAP-tag for superior tracking capabilities.
The study focuses on intracellular labelling of proteins within genomically re-coded E. coli to improve tracking accuracy.
Improvements in genetic code expansion technology may streamline protein studies, with implications for cellular biology.

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Implication

Optimization of single-molecule tracking for proteins in E. coli, suggesting improvements in labelling technology.

Abstract

Single-molecule tracking (SMT) is a powerful tool for real-time studies of protein interactions in living cells. Dye-labelled SNAP-tag and HaloTag self-labelling proteins have simplified SMT significantly, due to their superior...

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Ilievski et al. (2025) studied this question.

synapsesocial.com/papers/6928f126a65b730b9ea7a3ed https://doi.org/10.1039/d5cb00221d

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Optimization of the genetic code expansion technology for intracellular labelling and single-molecule tracking of proteins in genomically re-coded E. coli

Key Points

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Implication

Abstract

Discussion

Cite This Study

Optimization of the genetic code expansion technology for intracellular labelling and single-molecule tracking of proteins in genomically re-coded E. coli

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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