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November 30, 2025Nature Communications3 citationsOpen Access

Three-dimensional holographic imaging of incoherent objects through scattering media

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YBYoonSeok BaekHAHilton B. de AguiarSGSylvain Gigan

Key Points

  • 3D reconstructions achieved in challenging scattering media, enhancing imaging techniques.
  • Experimental validation with fluorescent objects confirms a robust non-invasive approach for imaging.
  • Utilizing spatial correlations, the method overcomes light scattering challenges in microscopy environments.
  • This approach may enable advanced applications in high-resolution imaging of complex biological samples.

Abstract

Abstract Three-dimensional (3D) high-resolution imaging is essential in microscopy, yet light scattering poses significant challenges in achieving it. Here, we present an approach to holographic imaging of spatially incoherent objects through scattering media, utilizing a virtual medium that replicates the scattering effects of the actual medium. This medium is constructed by retrieving mutually incoherent fields from the object and exploiting the spatial correlations between them. By numerically propagating the incoherent fields through the virtual medium, we non-invasively compensate for scattering, achieving accurate 3D reconstructions of hidden objects. Experimental validation with fluorescent and synthetic incoherent objects confirms the effectiveness of this approach, opening new possibilities for advanced 3D high-resolution microscopy in scattering environments.

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Cite This Study

Baek et al. (2025) studied this question.

synapsesocial.com/papers/692b944c1d383f2b2a378d9chttps://doi.org/10.1038/s41467-025-66626-7
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