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Evaluation of five preservation methods for fecal metaproteome, suggesting better protocols for gut microbiome analysis.
CAMPI-2 allowed a robust evaluation of five different methods for preserving fecal metaproteome samples. The present investigation provides useful data for the design of metaproteomics and multi-omics studies where fecal sampling cannot be immediately followed by long-term storage at - 80 °C. Further optimization of the tested protocols is necessary to improve stabilization efficiency and control bias in the taxonomic and functional profile of the gut microbiome. Video Abstract.
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Schallert et al. (2025) studied this question.
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