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This is a In Vitro Study study.

December 4, 2025BiomoleculesOpen Access

Optimized Method for Establishing Primary Human Mesothelial Cell Cultures Preserving Epithelial Phenotype

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Establishment of primary cultures increased cell survival, facilitating research on mesothelial cells.
Key metrics of cell survival were observed, with advancements in maintaining epithelial phenotype.
This method involved a combined protocol utilizing collagen IV, conditioned medium, and a ROCK inhibitor treatment.
Findings highlight the significance of optimized protocols for investigating therapeutic strategies against related diseases.

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Proposed method improves cell survival of mesothelial cells, indicating potential advances in therapeutic strategies for inflammation and fibrosis.

И. Б. Белоглазова (2025) studied this question.

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Proposed method improves cell survival of mesothelial cells, indicating potential advances in therapeutic strategies for inflammation and fibrosis.