Recombinant oz virus exhibits comparable replication kinetics to wild-type oz virus, hinting at its utility for molecular studies.
Oz virus (OZV) is a tick‐borne, six‐segmented, negative‐strand RNA virus in the genus Thogotovirus , family Orthomyxoviridae . A fatal human infection was reported in Japan in 2023. In this study, we established a reverse genetics system to generate infectious recombinant OZV. Six plasmids encoding the full‐length OZV genome segments under the control of a murine RNA polymerase I promoter, together with four plasmids expressing viral proteins essential for polymerase activity, were cotransfected into murine cells. This approach enabled efficient recovery of infectious OZV. The recovered recombinant virus exhibited replication kinetics comparable to wild‐type OZV. This system provides a platform for molecular studies of OZV.
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Rakib et al. (2025) studied this question.
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