Findings show that tumor associated macrophages promote an immunosuppressive phenotype via IL-10 in cutaneous DLBCL, enhancing T-cell inhibition and tumor survival.
Key Points
This research aims to examine the interplay between PCDLBCL-LT tumor B-cells and macrophages, focusing on immunosuppressive factors.
Utilized ARSI cell line and PCDLBCL-LT patient-derived xenografts for experiments.
Primary monocytes from healthy donors and THP-1 cell line were differentiated for macrophage studies.
Conducted flow cytometry and RNAseq to analyze macrophage changes and gene expression.
Performed LegendPlex assays to measure cytokine levels and observed T-cell responses to activated macrophages.
ARSI cell line increased CD163 and PD-L1 expression in macrophages, indicating an immunosuppressive phenotype.
RNAseq revealed differential gene expression promoting a C1Q signature associated with immune functions.
Increased IL-10 levels in macrophages co-cultured with ARSI were confirmed to support T-cell inhibition.
Macrophage changes were linked to specific tumor genetics, particularly MYD88 mutations.