Cohort study reveals dysregulated immune response affecting T cell differentiation in children with aplastic anemia, indicating potential immunotherapy pathways.
Key Points
The aim is to investigate the role of DNA methylation variability in T cell differentiation in pediatric aplastic anemia.
Cohort comparison of 76 children with aplastic anemia and 20 healthy controls
Utilized multi-omics approach including flow cytometry, whole genome bisulfite sequencing
Conducted single-cell RNA sequencing for T cell characterization
Increased T cell proportions and higher Th17/Treg ratios observed in AA patients
Hypomethylated STAT3 genes linked to enhanced Th17 cell differentiation
Dysregulation of IL-6/JAK2/STAT3 signaling pathway associated with T cell subset expansion