Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
December 8, 2025Blood

Pre-treatment immune profiling by flow cytometry and single-cell transcriptomics identifies distinct features in B and T cells in rituximab responders and non-responders

View Full Paper
Ask AI
Bookmark
Share

Authors

AAAlicia K. AuAKAkhil KheraAMAmna Malik

Discussion

Loading...

Member takes

Overview

Flow cytometry identifies immune activation in patients with ITP, suggesting biomarkers for rituximab response.

Key Points

  • This research aims to identify immune signatures associated with rituximab response in immune thrombocytopenia (ITP).
  • Clinical samples were collected from patients treated with rituximab for ITP.
  • Multi-parameter flow cytometry was performed on blood samples to assess immune subsets.
  • Single-cell RNA and TCR sequencing were conducted to analyze B and T cell profiles.
  • Complete responders (CRs) demonstrated an activated B cell profile, while non-responders (NRs) showed chronic immune activation.
  • NRs had higher levels of immune markers like CD95 and HLA-DR and displayed reduced BCR activity.
  • T cell clonal expansion and reduced diversity were observed in non-responders, indicating sustained T cell activation.

Cite This Study

Au et al. (2025) studied this question.

synapsesocial.com/papers/69362f364fa91c937236d307https://doi.org/10.1182/blood-2025-734
View Full Paper
Ask AI
Bookmark
Share