Single-cell transcriptomics reveals proliferative, immune-evasive, and bone marrow–independent transcriptional programs in extramedullary multiple myeloma.
Single-cell RNA sequencing identified proliferative and immune-evasive transcriptional programs in extramedullary multiple myeloma, suggesting independence from bone marrow.
Key Points
This research aims to uncover distinct transcriptional states in extramedullary multiple myeloma using single-cell RNA sequencing.
Analyzed clonal plasma cells from extramedullary disease and solitary plasmacytomas using single-cell RNA sequencing.
Utilized the 10x Genomics FLEX platform and NovaSeq X for sequencing 10,000–40,000 cells per library.
Applied RPCA in Seurat for data normalization and integration, assessing plasma cell identity and clonality.
Identified eight distinct cell clusters with significant differences in gene expression between EMD, SPM, and BMA.
Found consistent upregulation of the cell cycle and E2F pathways in EMD, indicating heightened proliferation.
Documented a downregulation of immune signaling and metabolism in EMD, consistent with immune escape and metabolic changes.