Comparative analysis reveals DNA sequencing identifies more fusion genes in non-Hodgkin lymphoma, highlighting its diagnostic superiority over RNA sequencing.
Key Points
Evaluate the concordance between DNA and RNA sequencing for detecting gene fusions in lymphoma.
Analyzed paired DNA-seq and RNA-seq data from non-Hodgkin lymphoma patients.
Used STAR-Fusion and Arriba for RNA-seq analysis; LUMPY for DNA-seq.
Manually confirmed candidate gene fusions with Integrative Genomics Viewer.
DNA sequencing detected 92% of fusion genes compared to 44% by RNA sequencing.
22 NHL cases showed a total of 25 fusion genes discovered with significant recurrent fusions.
DNA sequencing excelled in identifying intergenic breakpoints and cases with low-quality samples.