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December 8, 2025Blood

A novel method for molecular subtyping diffuse-large B-cell lymphoma using whole-exome sequencing

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Authors

YHYuefan Huang

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Overview

Novel method demonstrates significant association between molecular subtypes and ctDNA dynamics in DLBCL patients, indicating improved personalized monitoring.

Key Points

  • To develop a novel method for molecular subtyping of diffuse large B-cell lymphoma using whole-exome sequencing data.
  • Analyzed WES data from 200 DLBCL patients for molecular clustering.
  • Applied PAM clustering and LASSO regularization to identify mutation-defined subgroups.
  • Used Chi-square tests to correlate molecular clusters with ctDNA dynamics.
  • Identified distinct molecular clusters based on mutation frequency cutoffs using WES.
  • Certain subtypes showed significant association with ctDNA positive or negative results.
  • Developed methodology demonstrates potential for improved personalized monitoring and treatment selection.

Cite This Study

Yuefan Huang (2025) studied this question.

synapsesocial.com/papers/69362f484fa91c937236d671https://doi.org/10.1182/blood-2025-4336
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