Doxycycline-induced KMT2A-MLLT3 expression in a mouse model reveals critical cellular interactions in infant AML.
Key Points
This research aims to characterize the leukaemic microenvironment in a mouse model of infant acute myeloid leukaemia (AML) with KMT2A-MLLT3 expression.
Utilized a doxycycline-inducible mouse model to induce KMT2A-MLLT3 expression from embryonic day 12.5.
Performed single-cell RNA-sequencing (scRNA-Seq) on bone marrow of control and leukemic mice.
Characterized multiple stromal and endothelial cell populations within the bone marrow.
Identified a loss of stromal sub-populations and emergence of a fibroblast-like population in AML.
Noted severe depletion of secretory stromal populations that support haematopoietic cells.
Determined that KMT2A-MLLT3 blasts are associated with specific stromal populations and involved in immunosuppressive interactions.