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December 8, 2025Blood

Beyond complement: Patient and in vitro evidence for alternative mechanisms of Monocyte–Platelet aggregation in PNH

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Authors

FRFrederic Ries

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Overview

Observations reveal alternative mechanisms of platelet aggregation in paroxysmal nocturnal haemoglobinuria, suggesting new therapeutic approaches.

Key Points

  • This study aims to explore the mechanisms of monocyte-platelet aggregation in patients with paroxysmal nocturnal haemoglobinuria (PNH).
  • Analyzed peripheral blood samples from 13 PNH patients and 13 controls using flow cytometry
  • Identified monocytes using CD64 and distinguished PNH clones by loss of CD14
  • Generated a GPI-deficient monoctyic cell line (PIGA knockout) for aggregation assays
  • Performed aggregation assays with various stimulation conditions
  • Established a co-culture model of wild-type and GPI-deficient cells
  • WT-monocytes in PNH showed 16% platelet aggregation vs 6% in clonal PNH and 4.3% in controls
  • In vitro assays showed no increased aggregation with patient or control serum
  • Co-culture suggested thrombogenic activation by wild-type cells impacting GPI-deficient monocytes

Cite This Study

Frederic Ries (2025) studied this question.

synapsesocial.com/papers/69362f514fa91c937236d9bahttps://doi.org/10.1182/blood-2025-2988
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