Bulk and single cell RNA-sequencing reveals megakaryocyte phenotype changes in response to immune stimuli and infections including IFNγ and Plasmodium Yoelii.
Key Points
The study examines how transcription factor Myb regulates megakaryocyte phenotypes in response to immune and inflammatory signals.
Bulk and single cell RNA-sequencing of lung and bone marrow megakaryocytes was conducted.
A GFP-Myb reporter mouse model was used to track Myb expression changes.
Interferon gamma injections and Plasmodium Yoelii infections tested immune responses.
Flow cytometry analyzed phenotypic markers in megakaryocytes and platelets.
Myb expression in bone marrow megakaryocytes decreased following IFNγ treatment and Plasmodium Yoelii infection.
Megakaryocytes without Myb exhibited increased ICAM1, MHCII, and TLR7 expression.
Platelets from Myb negative megakaryocytes had enhanced activation and leukocyte aggregation under TLR7 stimulation.