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December 9, 2025Proceedings of the National Academy of Sciences1 citations

Dynamics of T7 RNAP Transcription Studied Through Single-Molecule Imaging

Heterogeneous template-dependent transcription dynamics of T7 RNAP revealed by single-molecule imaging

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Authors

SZSarah ZerniaJDJoëlle Deplazes-LauberJHJonas Huber

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Overview

Single-molecule imaging reveals T7 RNAP elongation dynamics and recognition motifs impacting transcription outcomes.

Key Points

  • This research aims to understand the transcription dynamics of T7 RNAP through single-molecule imaging techniques.
  • Utilized single-molecule high-throughput transcription assay with DNA curtains to study T7 RNAP dynamics.
  • Investigated initiation, elongation, pausing, and termination of transcription at different promoter sites.
  • Analyzed the effects of DNA shape parameters and dimethyl sulfoxide on transcription initiation.
  • Identified common impediments affecting T7 RNAP performance during transcription initiation and elongation.
  • Discovered two types of sequence-dependent pauses, differentiating between short and long pause types.
  • Showed that dimethyl sulfoxide can improve transcription initiation under suboptimal conditions.

Cite This Study

Zernia et al. (2025) studied this question.

synapsesocial.com/papers/69401d732d562116f28f94fbhttps://doi.org/10.1073/pnas.2514125122
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