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December 3, 2025Optics ExpressOpen Access

Modeling and optimization of time correlated single photon counting accuracy in fluorescence lifetime measurements using delay control

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Authors

JRJonas RottmannJDJeng-Cherng DungNSNicolas Schierbaum

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Overview

Theoretical model enhances photon counts in fluorescence lifetime measurements, indicating improved accuracy at high saturation levels.

Key Points

  • Accuracy improved in fluorescence lifetime measurements with a single photon avalanche diode.
  • This novel delay method enhances photon counts, achieving accuracy within ±15% during high detector saturation.
  • Theoretical model assessing the detection process was applied to optimize fluorescence lifetime measurements.
  • Findings suggest a potential shift in measurement practices for robust and fast fluorescence lifetime results.

Cite This Study

Rottmann et al. (2025) studied this question.

synapsesocial.com/papers/694025792d562116f28fdfe0https://doi.org/10.1364/oe.579675
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