Using integrated cpDNA and ITS markers, we established optimized core collections for both species (N. incisum: 50-103 accessions; N. franchetii: 30-40 accessions) that effectively preserved genetic diversity. The dual-marker approach resolved cultivated populations' paradoxical genetic patterns (higher diversity but lower differentiation) and provides a conservation model for medicinal plants facing anthropogenic pressures.
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Yang et al. (2025) studied this question.
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