Protocol to study social transmission of stress in group-housed mice using restraint stress

Here, we present a protocol to study social transmission of stress in group-housed mice using restraint stress. We describe steps for exposing mice to restrained conspecifics separated by an acrylic divider to induce indirect stress responses. We detail procedures for acute and chronic paradigms, followed by behavioral, physiological, and immunological assessments in both restrained and bystander mice. The protocol allows for the investigation of sensory and social mechanisms underlying stress contagion, while excluding visual cues. For complete details on the use and execution of this protocol, please refer to Horvath et al.¹.