Identification of Acinetobacter baumannii based on plasmonic patterns of silver nanoprism reinforced with 3,3′,5,5′-tetramethylbenzidine and hydrogen peroxide: An innovative DNA-based optical biosensor

In this study, silver nanoprisms (AgNPrs) was utilized as optical probe to create advanced DNA-based biosensor (genosensor) for detecting target sequences (cDNA) of A. baumannii using oligonucleotides hybridization strategy. The engineered genosensor demonstrated exceptional sensitivity in the dynamic range from 10−18 to 10−6 M. The genosensor achieved a low limit of quantification of 10−18 M, making it a highly effective alternative to traditional detection methods. Additionally, specify of the biosensor's was assessed, particularly regarding two mismatch sequences, allowing precise measurement of A. baumannii without extensive preparation steps. Therefore, an efficient biosensor was developed for real-time monitoring and tracking in pathogen detection and introduced DNA-based biosensors as a powerful and next generation diagnostic approach of bacterial infections.