What question did this study set out to answer?

The research aims to explore how HLA-G1 overexpressing mesenchymal stromal cells affect immune response and heart recovery after myocardial infarction.

January 10, 2026Stem Cell Research & Therapy0 citationsOpen Access

Immunomodulatory effect of mesenchymal stromal cell overexpressing HLA-G1 in cell-based therapy for myocardial infarction

WZWei Zhu JKJie Kong HLHong-Xia Li

Key Result

HLA-G1 overexpressing hUCB-MSCs improved heart function after acute myocardial infarction by enhancing immune modulation and engraftment of transplanted hiPSC-CMs.

Key Points

The research aims to explore how HLA-G1 overexpressing mesenchymal stromal cells affect immune response and heart recovery after myocardial infarction.
Administered HLA-G1 overexpressing human umbilical cord blood mesenchymal stromal cells (hUCB-MSCs) intravenously.
Evaluated the immune response modulation and engraftment of transplanted cells.
Assessed improvement in heart function post-acute myocardial infarction (AMI).
HLA-G1 overexpressing hUCB-MSCs significantly modulated the immune response.
There was an increased engraftment of transplanted human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs).
Improvement in heart function was noted following treatment.

Structured PICO

Does intravenous administration of HLA-G1 overexpressing hUCB-MSCs improve the engraftment of intramyocardially transplanted hiPSC-CMs and cardiac function in a mouse model of acute myocardial infarction?

Population

30 male ICR mice (age 6 weeks, weight 18-22 g) with acute myocardial infarction (AMI) induced by ligating the left anterior descending coronary artery.

Intervention

Intravenous administration of HLA-G1 overexpressing human umbilical cord blood-derived mesenchymal stromal cells (hUCB-MSCs, 5 × 10^5) via tail vein 7 days before AMI induction, followed by intramyocardial injection of 1 × 10^6 human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) 30 min after AMI induction.

Comparator

Sham (intravenous saline + thoracotomy), AMI control (intravenous saline + AMI + intramyocardial culture medium), AMI + hiPSC-CMs (intravenous saline + AMI + intramyocardial hiPSC-CMs), and standard hUCB-MSCs (intravenous standard hUCB-MSCs + AMI + intramyocardial hiPSC-CMs).

Outcome

Cardiac function (LVEF, FS, LVEDD, LVESD) evaluated by transthoracic echocardiography on day 28.surrogate

In a mouse model of myocardial infarction, HLA-G1 overexpressing mesenchymal stromal cells enhanced immunomodulation, increased the engraftment of transplanted cardiomyocytes, and significantly improved left ventricular systolic function.

Main Result

Absolute Event Rate: 0% vs 0%

Abstract

This study showed that systemic intravenous administration of HLA-G1 overexpressing hUCB-MSCs modulated immune response and increased transplanted hiPSC-CMs' engraftment to improve heart function following AMI.

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