What question did this study set out to answer?

The study aims to evaluate the relationship between soluble neprilysin (sNEP) and myocardial damage in a doxorubicin-induced cardiotoxicity model.

January 10, 2026Cardio-Oncology0 citationsOpen Access

Soluble neprilysin is associated with myocardial damage and systolic dysfunction in an animal model of doxorubicin-induced cardiotoxicity

ASAleksandra SobiborowiczMedical University of Warsaw KKKatarzyna KamińskaHeart Failure / Cardiomyopathy DSDorota SztechmanMedical University of Warsaw

Key Result

Elevated serum soluble neprilysin (sNEP) is associated with greater cardiomyocyte injury and more severe cardiac output decline in doxorubicin-induced cardiotoxicity.

Key Points

The study aims to evaluate the relationship between soluble neprilysin (sNEP) and myocardial damage in a doxorubicin-induced cardiotoxicity model.
Animal model of doxorubicin exposure used to study cardiac effects
Measurement of left ventricular neprilysin (NEP) protein expression
Assessment of serum sNEP levels
Evaluation of cardiac output and cardiomyocyte injury
Chronic doxorubicin exposure reduces left ventricular neprilysin protein expression
Elevated serum sNEP levels correlate with early cardiomyocyte injury
In chronic settings, higher sNEP levels relate to significant declines in cardiac output

Structured PICO

Does doxorubicin alter neprilysin expression and do sNEP levels correlate with myocardial damage in a rat model of anthracycline-induced cardiotoxicity?

Population

35 male 12-week-old Sprague-Dawley rats (acute model: n=8 DOX, n=8 control; chronic model: n=11 DOX, n=8 control)

Intervention

Doxorubicin intraperitoneal injection (Acute model: single 20 mg/kg dose; Chronic model: four weekly doses of 5 mg/kg)

Comparator

Normal saline intraperitoneal injection (corresponding volume)

Outcome

Neprilysin (NEP) protein and mRNA expression in left ventricular tissue, and soluble NEP (sNEP) concentrations in serumsurrogate

Chronic doxorubicin exposure reduces LV neprilysin protein expression, and elevated serum soluble neprilysin correlates with early cardiomyocyte injury and chronic decline in cardiac output, suggesting its potential as a biomarker for anthracycline-induced cardiotoxicity.

Main Result

Absolute Event Rate: 0% vs 0%

Abstract

Chronic DOX exposure reduces LV NEP protein expression. Elevated serum sNEP is associated with greater early cardiomyocyte injury, while in chronic AIC, it correlates with a more severe decline in cardiac output. These findings suggest sNEP may be a potential biomarker for AIC.

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