Cardionogen-1, a novel small molecule, induces cytotoxicity by inhibiting Wnt/β-catenin signalling pathway in Huh-7 cells

Key Result

Cardionogen-1 induced cytotoxicity in Huh-7 cells by inhibiting Wnt/β-catenin signaling, increasing apoptosis markers and disrupting the cell cycle in a dose-dependent manner.

Key Points

• To evaluate the cytotoxic effects of cardionogen-1 on human hepatoma Huh-7 cells by inhibiting the Wnt/beta-catenin signaling pathway.
• Treated Huh-7 cells with cardionogen-1 at varying concentrations for 24 hours.
• Conducted cytotoxicity assays and analyzed reactive oxygen species levels.
• Evaluated apoptosis through flow cytometry and fluorescence microscopy.
• Used PCR and western blotting to assess protein expression changes associated with apoptosis.
• Cardionogen-1 significantly decreased the viability and proliferation of Huh-7 cells in a dose-dependent manner.
• Induced early apoptosis characterized by increased ROS levels and activation of pro-apoptotic proteins like p53 and Bax.
• Reduced levels of the anti-apoptotic protein Bcl-2 were observed, indicating enhanced apoptotic processes.
• Disrupted the cell cycle, leading to accumulation of cells in the G phase.