What question did this study set out to answer?

This research aims to evaluate the expression levels of miR-377-3p in serum and its diagnostic potential for carotid artery stenosis.

January 10, 2026International Journal of General Medicine0 citationsOpen Access

Serum Aberrant Expression of miR-377-3p and Its Diagnostic Value in Carotid Artery Stenosis

Q: What does this research mean for the field?

Serum miR-377-3p levels are significantly lower in carotid artery stenosis patients, demonstrating high diagnostic value with an AUC of 0.9597. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

GQGuohua QieHebei North University HHHuixian Han JNJianfeng NingWeierstrass Institute for Applied Analysis and Stochastics

Key Result

Serum miR-377-3p levels were significantly lower in carotid artery stenosis patients, demonstrating high diagnostic value with an AUC of 0.9597.

Key Points

This research aims to evaluate the expression levels of miR-377-3p in serum and its diagnostic potential for carotid artery stenosis.
Cross-sectional study design
Analysis of serum miR-377-3p levels
Comparison with standard diagnostic markers for carotid artery stenosis
Higher levels of miR-377-3p correlate with increased carotid artery stenosis severity
miR-377-3p shows potential diagnostic sensitivity and specificity for detecting stenosis
Significant differences in miR-377-3p levels between stenosis patients and controls

Structured PICO

Does serum miR-377-3p have diagnostic value for carotid artery stenosis?

Population

156 participants including 78 patients with carotid artery stenosis (≥50% stenosis, aged 50-75 years) and 78 age- and gender-matched healthy controls, as well as human aortic smooth muscle cells (HASMCs) for in vitro experiments.

Comparator

Healthy controls with <10% stenosis and no risk factors for carotid artery stenosis.

Outcome

Diagnostic value of serum miR-377-3p for carotid artery stenosis assessed by ROC curve area under the curve (AUC).surrogate

Serum miR-377-3p is significantly downregulated in patients with carotid artery stenosis and demonstrates high diagnostic accuracy, while also inhibiting vascular smooth muscle cell proliferation and migration by targeting EDIL3.

Main Result

Absolute Event Rate: 0% vs 0%

Abstract

Purpose: To determine the serum expression profile of miR-377-3p in carotid artery stenosis (CAS) patients and to assess its diagnostic potential in a clinical setting. Patients and Methods: Using qRT-PCR, miR-377-3p expression was measured in serum samples from 78 CAS patients and 78 matched healthy controls. We used the Pearson correlation to analyze inter-indicator relationships and the ROC curve to assess the diagnostic significance of miR-377-3p. The cellular functions of human aortic smooth muscle cells (HASMCs) were assessed. Bioinformatics predictions of target associations were experimentally verified with a luciferase activity assay. Results: Patients diagnosed with CAS had a lower serum level of miR-377-3p. ROC curve-based analysis showed that miR-377-3p exerted high diagnostic value, where the area under the curve (AUC) was 0.9597. Serum miR-377-3p was negatively correlated with systolic blood pressure (SBP), diastolic blood pressure (DBP), fasting blood glucose (FBG), and low-density lipoprotein cholesterol (LDL), while positively correlated with high-density lipoprotein cholesterol (HDL). miR-377-3p inhibited the proliferation and migration of HASMCs, but this effect was counteracted by EDIL3. Conclusion: These results suggest the dual utility of miR-377-3p as a promising biomarker and a potential therapeutic target in the management of CAS. miR-377-3p can inhibit the proliferation and migration of HASMCs by targeting EDIL3.

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